(KO Validated) TFAP4 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88666A
Western blot analysis of extracts of various cell lines using TFAP4 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts from normal (control) and TFAP4 knockout (KO) HeLa cells using TFAP4 Polyclonal Antibody at 1:500 dilution.
Immunofluorescence analysis of C6 cells using TFAP4 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using TFAP4 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
(KO Validated) TFAP4 Polyclonal Antibody
Catalog Number
RD88666A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM, Jul 2009]
Immunogen Information
Immunogen
A synthetic peptide of human TFAP4
Gene ID
7023
Swissprot
Q01664
Synonyms
TFAP4AP-4bHLHc41
Calculated MW
55kDa/57kDa
Observed MW
57kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:200-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.