(KO Validated) TOM20 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90233A
Western blot analysis of extracts of various cell lines using TOM20 Polyclonal Antibody at 1:3000 dilution.
Western blot analysis of extracts of various cell lines using TOM20 Polyclonal Antibody at 1:3000 dilution.
Western blot analysis of extracts from normal (control) and TOM20 knockout (KO) 293T cells using TOM20 Polyclonal Antibody at 1:3000 dilution.
![Immunofluorescence analysis of C6 cells using [KO Validated] TOM20 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90233A-IF01.jpg)
Immunofluorescence analysis of C6 cells using [KO Validated] TOM20 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] TOM20 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90233A-IF02.jpg)
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] TOM20 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of U2OS cells using [KO Validated] TOM20 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90233A-IF03.jpg)
Immunofluorescence analysis of U2OS cells using [KO Validated] TOM20 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
(KO Validated) TOM20 Polyclonal Antibody
Catalog Number
RD90233A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Immunogen Information
Immunogen
Recombinant fusion protein of human TOMM20
Gene ID
9804
Swissprot
Q15388
Synonyms
TOMM20MAS20MOM19TOM20
Calculated MW
20kDa/60kDa
Observed MW
70KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas