(KO Validated) TYSY Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89442A
Western blot analysis of extracts from wild type(WT) and TYSY knockout (KO) 293T cells, using TYSY Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse spleen using TYMS Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using TYMS Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using TYMS Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using TYMS Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
(KO Validated) TYSY Polyclonal Antibody
Catalog Number
RD89442A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
Immunogen Information
Immunogen
Recombinant fusion protein of human TYSY
Gene ID
7298
Swissprot
P04818
Synonyms
TYMSHST422TMSTSthymidylate synthase
Calculated MW
8kDa/11kDa
Observed MW
16KDa/80KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.