(KO Validated) Vimentin Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using Vimentin Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using Vimentin Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells using Vimentin Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells using Vimentin Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human tonsil using Vimentin Polyclonal Antibody at dilution of  1:150 (40x lens).
Immunohistochemistry of paraffin-embedded Human tonsil using Vimentin Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded Human stomach using Vimentin Polyclonal Antibody at dilution of  1:150 (40x lens).
Immunohistochemistry of paraffin-embedded Human stomach using Vimentin Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunofluorescence analysis of L929 cells using Vimentin Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using Vimentin Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
(KO Validated) Vimentin Polyclonal Antibody
Catalog Number
RD80735A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human Vimentin (NP_003371.2).
Gene ID
7431
Swissprot
P08670
Synonyms
CTRCT30HEL113VimentinVIMvimentin
Calculated MW
53 kDa
Observed MW
57 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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