Acetyl-P53 (Lys381) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86211A
Western Blot analysis of HepG2, 293T-UV, Hela, AD293T, Hela-UV cells using p53 (Acetyl Lys381) Polyclonal Antibody at dilution of 1:1000.
Product Name
Acetyl-P53 (Lys381) Polyclonal Antibody
Catalog Number
RD86211A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Immunogen Information
Immunogen
Synthesized peptide derived from human p53 around the acetylation site of K381
Swissprot
P04637
Synonyms
Antigen NY-CO-13BCC7Cellular tumor antigen p53FLJ92943LFS1Mutant tumor protein 53p53p53 tumor suppressorP53Phosphoprotein p53Tp53Transformation related protein 53TRP53Tumor protein 53Tumor protein p53Tumor suppressor p53
Calculated MW
44 kDa
Observed MW
53 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas