For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88528A
Western blot analysis of extracts of various cell lines using ACSL1 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using ACSL1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ACSL1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extracts of Raji cells using 3ug ACSL1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using ACSL1 Polyclonal Antibody at a dilution of 1:1000.
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human ACSL1
Gene ID
2180
Swissprot
P33121
Synonyms
ACS1FACL1FACL2LACSLACS1LACS2ACSL1
Calculated MW
49kDa/50kDa 56kDa/73kDa
Observed MW
110kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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