Active CASP3 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85535A
Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain with Active CASP3 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded Human liver tissue using Active CASP3 Monoclonal Antibody at dilution of 1:200.
Product Name
Active CASP3 Monoclonal Antibody
Catalog Number
RD85535A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
Immunogen Information
Immunogen
Recombinant Protein
Swissprot
P42574
Synonyms
A830040C14RikApopainCASP-3CASP3CASP3Casp3aCaspase 3Caspase 3apoptosis-related cysteine peptidaseCaspase 3apoptosis-related cysteine proteaseCaspase 3apoptosis-related cysteine protease aCaspase-3 subunit p12CC3CPP-32CPP32CPP32BCysteine p
Observed MW
17 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:100-1:200, IF 1:100-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas