AF488 Anti-Mouse CD45.2 Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD21540F
. Unstained splenocytes (empty black histogram) are used as control.](/upload/ec/antibodies/RD21540F-FC01.jpg)
C57BL/6 murine splenocytes are stained with Anti-Mouse CD45.2 Monoclonal Antibody(AF488 Conjugated)[Used at 0.2 μg/106 cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.
Variation
Quantity
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Overview
Product Name
AF488 Anti-Mouse CD45.2 Antibody
Catalog Number
RD21540F
Clonality
Monoclonal
Isotype
Mouse IgG2a, κ
Host
Mouse
Background
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Immunogen Information
Gene ID
19264
Synonyms
Ly-5.2LCA
Applications
Reactivity
Mouse
Tested Applications
FCM
Conjugation
AF488
Concentration
0.5 mg/mL
Storage Buffer
PBS with 0.05% Proclin300, 1% BSA
Storage Instructions
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.