AF488 Anti-Rat CD8a Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD21173F
Rat splenocytes are  stained with Anti-Rat CD8a Monoclonal Antibody(AF488 Conjugated)[Used at 0.2 μg/10<sup>6</sup> cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.
Rat splenocytes are stained with Anti-Rat CD8a Monoclonal Antibody(AF488 Conjugated)[Used at 0.2 μg/106 cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.
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Overview

Circle
Product Name
AF488 Anti-Rat CD8a Antibody
Catalog Number
RD21173F
Clonality
Monoclonal
Isotype
Mouse IgG1, κ
Host
Mouse

Background

Circle
CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Immunogen Information

Circle
Gene ID
24930
Swissprot
P07725
Synonyms
T-cell surface glycoprotein CD8 alpha chainCD8AT-lymphocyte differentiation antigen T8/Leu-2MAL

Applications

Circle
Reactivity
Rat
Tested Applications
FCM
Conjugation
AF488
Concentration
0.5 mg/mL
Storage Buffer
PBS with 0.05% Proclin300, 1% BSA
Storage Instructions
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.

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