For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD21225F
C57BL/6 murine splenocytes are stained with Anti-Mouse CD25 Monoclonal Antibody(AF647 Conjugated)[Used at 0.2 μg/106 cells dilution]and Anti-Mouse CD4 Monoclonal Antibody(FITC Conjugated). Splenocytes stained with Anti-Mouse CD4 Monoclonal Antibody(FITC Conjugated)and Rat IgG1 Isotype Control(AF647 Conjugated)(right) are used as control.
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.