C57BL/6 murine splenocytes are stained with FITC Anti-Mouse CD3 Antibody and AF 647 Anti-Mouse CD73 Antibody (Left). Splenocytes are stained with FITC Anti-Mouse CD3 Antibody and AF 647 Rat IgG2a, κ Isotype Control (Right).
CD73 (ecto-5`-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.