AF647 Anti-Mouse F4/80 Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD20142F
. Unstained macrophages (blank black histogram) are used as control.](/upload/ec/antibodies/RD20142F-FC01.jpg)
Mouse abdominal macrophages elicited by starch broth are stained with Anti-Mouse F4/80 Monoclonal Antibody(AF647 Conjugated)[Used at 0.2 μg/106 cells dilution](filled gray histogram). Unstained macrophages (blank black histogram) are used as control.
Product Name
AF647 Anti-Mouse F4/80 Antibody
Catalog Number
RD20142F
Clonality
Monoclonal
Isotype
Rat IgG2b, κ
Host
Rat
Background
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
Immunogen Information
Gene ID
13733
Swissprot
Q61549
Synonyms
Adhesion G protein-coupled receptor E1Adgre1Cell surface glycoprotein F4/80EGF-like module receptor 1Adgre1Emr1Gpf480
Applications
Reactivity
Mouse
Tested Applications
FCM
Conjugation
AF647
Concentration
0.5 mg/mL
Storage Buffer
PBS with 0.05% Proclin300, 1% BSA
Storage Instructions
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Research Areas