C57BL/6 murine splenocytes are stained with FITC Anti-Mouse CD8a Antibody and AF Violet 450 Anti-Mouse CD4 Antibody[RM4-5] (Left). Splenocytes are stained with FITC Anti-Mouse CD8a Antibody and AF Violet 450 Rat IgG2a, κ Isotype Control (Right).
CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.