For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90083A
Western blot analysis of extracts of mouse brain using Aggrecan Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of Mouse cartilage cells using Aggrecan Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Rat cartilage cells using Aggrecan Polyclonal Antibody at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human Aggrecan
Gene ID
176
Swissprot
P16112
Synonyms
ACANAGC1AGCANCSPG1CSPGCPMSK16SEDKaggrecan
Calculated MW
43kDa/45kDa
Observed MW
45kDa
Applications
Reactivity
Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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