For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89851A
Western blot analysis of extracts of Mouse skeletal muscle using AGRP Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse adrenal gland using AGRP Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity.
Immunogen Information
Immunogen
Recombinant fusion protein of human AGRP
Gene ID
181
Swissprot
O00253
Synonyms
AGRPAGRTARTASIP2
Calculated MW
25kDa/28kDa/34kDa/39kDa
Observed MW
39KDa
Applications
Reactivity
Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.