AHNAK Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89306A
Immunohistochemistry of paraffin-embedded Mouse liver using AHNAK Polyclonal Antibody at dilution of 1:50 (40x lens).1.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse brain using AHNAK Polyclonal Antibody at dilution of 1:50 (40x lens).1.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using AHNAK Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A-549 cells using AHNAK Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
AHNAK Polyclonal Antibody
Catalog Number
RD89306A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. A much shorter variant encoding a 17 kDa isoform exists for this gene, and the shorter isoform initiates a feedback loop that regulates alternative splicing of this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human AHNAK
Gene ID
79026
Swissprot
Q09666
Calculated MW
65kDa
Observed MW
70KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IHC,IF
Conjugation
Unconjugated
Dilution
IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.