AKR1C2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD265772A
Western blot analysis of extracts of various cell lines using AKR1C2 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of L929 cells using AKR1C2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using AKR1C2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
AKR1C2 Polyclonal Antibody
Catalog Number
RD265772A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human AKR1C2 (NP_995317.1).
Gene ID
1646
Swissprot
P52895
Synonyms
AKR1C2AKR1C-pseudoBABPDDDD-2DD/BABPDD2DDH2HAKRDHBABMCDR2SRXY8TDD
Calculated MW
15 kDa/36 kDa
Observed MW
37 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas