This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation. Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites.
Immunogen Information
Immunogen
Recombinant protein of human ALKBH1
Swissprot
Q13686
Synonyms
ABHABH1alkBalkB homolog 1 histone H2A dioxygenaseAlkBalkylation repair homolog 1 (E. coli)ALKB1ALKBHALKBH1Alkylated DNA repair protein alkB homolog 1Alkylation repair homolog 1Alkylation repairalkB homologAlpha-ketoglutarate-dependent dioxyge
Calculated MW
44 kDa
Gene Accession
BC025787
Applications
Reactivity
Human,Mouse
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
0.3 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4