ALKBH2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD75415A
Western Blot analysis of Human fetal liver and seminoma tissue, RAW264.7 cell using ALKBH2 Polyclonal Antibody at dilution of 1:800
Product Name
ALKBH2 Polyclonal Antibody
Catalog Number
RD75415A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine. Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
Immunogen Information
Immunogen
Recombinant protein of human ALKBH2
Swissprot
Q6NS38
Synonyms
2OG Fe(II) oxy DC19530023G02ABH 2ABH2alkB alkylation repair homolog 2ALKB2ALKBH2 alkBALKBH2Alkylated DNA repair protein alkB homologAlkylated DNA repair protein alkB homolog 2Alkylation repair homolog 2Alpha ketoglutarate dependent dioxygenase
Calculated MW
29 kDa
Gene Accession
BC070489
Applications
Reactivity
Human,Mouse
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
0.3 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas