ALKBH5 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90075A
Western blot analysis of extracts of various cell lines using ALKBH5 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse lung using ALKBH5 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat testis using ALKBH5 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Name
ALKBH5 Polyclonal Antibody
Catalog Number
RD90075A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6-methyladenosine (m6A RNA, the most prevalent internal modification of messenger RNA (mRNA in higher eukaryotes. Can also demethylate N(6-methyladenosine in single-stranded DNA (in vitro. Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity.
Immunogen Information
Immunogen
Recombinant fusion protein of human ALKBH5
Gene ID
54890
Swissprot
Q6P6C2
Synonyms
ALKBH5ABH5OFOXDOFOXD1
Calculated MW
48kDa/53kDa
Observed MW
48KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.