alpha Tubulin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86505A
Western Blot analysis of 1) Hela, 2) Rat brian, 3) Mouse brain using alpha Tubulin Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded mouse liver using alpha Tubulin Monoclonal Antibody at dilution of 1:200
Immunofluorescence analysis of Mouse liver tissue using alpha Tubulin Monoclonal Antibody at dilution of 1:200.
Product Name
alpha Tubulin Monoclonal Antibody
Catalog Number
RD86505A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
Immunogen Information
Immunogen
Recombinant Protein
Swissprot
Q71U36,P68363
Synonyms
TUBA1ATUBA3Tubulin alpha-1A chainAlpha-tubulin 3Tubulin B-alpha-1Tubulin alpha-3 chainTUBA1BTubulin alpha-1B chainAlpha-tubulin ubiquitousTubulin K-alpha-1Tubulin alpha-ubiquitous chain
Calculated MW
50 kDa
Observed MW
52 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-10000, IHC 1:50-300, IF 1:50-1:200, IP 1:100-1:300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas