alpha Tubulin Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86535A
Western Blot analysis of Jurkat cells using alpha Tubulin Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human brain tissue using alpha Tubulin Polyclonal Antibody at dilution of 1:100.
Product Name
alpha Tubulin Polyclonal Antibody
Catalog Number
RD86535A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
Immunogen Information
Immunogen
Synthesized peptide derived from human Tubulin α around the non-acetylation site of K40.
Swissprot
Q71U36
Synonyms
Alpha tubulin 3Alpha-tubulin 3B alpha 1FLJ25113LIS3TBA1ATUBA1ATUBA3Tubulin alpha 1aTubulin alpha 1A chainTubulin alpha 3Tubulin alpha 3 chainTubulin alpha brain specificTubulin alpha-1A chainTubulin alpha-3 chainTubulin B alpha 1Tubulin B-
Calculated MW
50 kDa
Observed MW
50 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000-1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas