AMPKβ1 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD89426A
Western blot analysis of extracts of MCF7 cells using AMPKβ1 Polyclonal Antibody at1:1000 dilution.
Western blot analysis of extracts of MCF7 cells using AMPKβ1 Polyclonal Antibody at1:1000 dilution.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

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Product Name
AMPKβ1 Polyclonal Antibody
Catalog Number
RD89426A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Immunogen Information

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Immunogen
A synthetic peptide of human AMPKβ1
Gene ID
5564
Swissprot
Q9Y478
Synonyms
PRKAB1AMPKHAMPKb5'-AMP-activated protein kinase subunit beta-1
Calculated MW
29kDa/30kDa
Observed MW
Refer to figures

Applications

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Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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