AMPK alpha1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85546A
Western Blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) PC-12 cells using AMPK alpha1 Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Mouse colon tissue using AMPK alpha1 Monoclonal Antibody at dilution of 1:200.
Product Name
AMPK alpha1 Monoclonal Antibody
Catalog Number
RD85546A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Immunogen Information
Immunogen
Synthetic Peptide
Swissprot
Q13131
Synonyms
5 AMP activated protein kinase alpha 1catalytic subunit5 AMP activated protein kinase catalytic alpha 1 chain5' AMP activated protein kinase catalytic subunit alpha 15'-AMP-activated protein kinase catalytic subunit alpha-1AAPK1AAPK1ACACA kinaseace
Observed MW
63 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p
Conjugation
Unconjugated
Dilution
WB 1:1000-2000, IHC 1:50-100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.