AMPK alpha2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90651A
Western blot with Ampk2 Polyclonal antibody at dilution of 1:1000.lane 1:Hep G2 whole cell lysate, lane 2:Hela whole cell lysate, lane 3:NIH/3T3 whole cell lysate
Immunohistochemistry of paraffin-embedded Human breast using AMPK alpha2 Polyclonal Antibody at dilution of 1:100
Immunofluorescence analysis of McF7 cells using AMPK alpha2 Polyclonal Antibody at dilution of 1:100
Immunofluorescence analysis of MCF7 cells using AMPK alpha2 Polyclonal Antibody at dilution of 1:100
Product Name
AMPK alpha2 Polyclonal Antibody
Catalog Number
RD90651A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Immunogen Information
Immunogen
Recombinant Rat Ampk2 protein expressed by E.coli
Swissprot
Q09137
Synonyms
5'-AMP-activated protein kinase catalytic subunit alpha-2AAPK2ACACA kinaseAcetyl-CoA carboxylase kinaseAMPK alpha 2 chainAMPK subunit alpha-2AMPK2AMPKa2AMPKalpha2HMGCR kinaseHydroxymethylglutaryl-CoA reductase kinasePRKAAPRKAA2Protein kinase
Calculated MW
15 kDa
Observed MW
15 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:100-1:200, IF 1:100-1:400
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas