AMPKα1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90337A
Immunofluorescence analysis of HeLa cells using AMPKα1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using AMPKα1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using AMPKα1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
AMPKα1 Polyclonal Antibody
Catalog Number
RD90337A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Immunogen Information
Immunogen
A synthetic peptide of human AMPKα1
Gene ID
5562
Swissprot
Q13131
Synonyms
PRKAA1AMPKAMPKa1protein kinase AMP-activated catalytic subunit alpha 1
Calculated MW
63kDa/92kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IF
Conjugation
Unconjugated
Dilution
IF 1:50-1:20
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.