ANKLE2 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD88894A
Western blot analysis of extracts of various cell lines using ANKLE2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using ANKLE2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Mouse kidney using ANKLE2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse kidney using ANKLE2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat testis using ANKLE2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Rat testis using ANKLE2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human lung cancer using ANKLE2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human lung cancer using ANKLE2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Overview

Circle
Product Name
ANKLE2 Polyclonal Antibody
Catalog Number
RD88894A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes a member of the LEM family of inner nuclear membrane proteins. The encoded protein functions as a mitotic regulator through postmitotic formation of the nuclear envelope. Mutations in this gene cause morphology defects in the nuclear envelope and BAF hyperphosphorylation. [provided by RefSeq, Mar 2014]

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human ANKLE2
Gene ID
23141
Swissprot
Q86XL3
Synonyms
KIAA0692LEMD7Lem4MCPH16ANKLE2
Observed MW
104kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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