For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90526A
Western Blot analysis of Recombinant MERS-CoV Nucleoprotein / NP protein (His Tag)(PKSV030235 with 500ng, 200ng and 100ng ) using Anti-MERS-CoV Nucleocapsid Protein Polyclonal Antibody at dilution of 1:1000.
Anti-MERS-CoV Nucleocapsid Protein Polyclonal Antibody
Catalog Number
RD90526A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Immunogen Information
Immunogen
A synthetic peptide corresponding to the center region of MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Protein (NP protein)