For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90544A
Western Blot analysis of Recombinant ZIKV (strain Zika SPH2015) Envelope protein (Domain III, His Tag)(PKSV030271 with 5ng) using Anti-Zika virus(ZIKV)(strain Zika SPH2015) ZIKV-E/Envelope protein(Domain III) Monoclonal Antibody at dilution of 1:2000.
Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Immunogen Information
Immunogen
Recombinant ZIKV (strain Zika SPH2015) Envelope protein (Domain III, His Tag)
Synonyms
EEnvelope Protein
Applications
Reactivity
Zika Virus
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:2000-1:10000
Concentration
1mg/mL
Storage Buffer
0.2 μm filtered solution in PBS
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Your item has been successfully added to your cart .