For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90061A
Western blot analysis of extracts of various cell lines using ANXA1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human liver cancer using [KO Validated] ANXA1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human tonsil using [KO Validated] ANXA1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of PC-12 cells using [KO Validated] ANXA1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using [KO Validated] ANXA1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.
Immunogen Information
Immunogen
Recombinant fusion protein of human ANXA1
Gene ID
301
Swissprot
P04083
Synonyms
ANXA1ANX1LPC1
Calculated MW
26kDa
Observed MW
26kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.