APAF1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD265705A
Western blot analysis of extracts of 293 cells using APAF1 Polyclonal Antibody.
Product Name
APAF1 Polyclonal Antibody
Catalog Number
RD265705A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Immunogen Information
Immunogen
Recombinant fusion protein of human APAF1 (NP_863651.1).
Gene ID
317
Swissprot
O14727
Synonyms
APAF1APAF-1CED4
Calculated MW
37 kDa/133 kDa/135 kDa/137 kDa/140 kDa/141 kDa
Observed MW
142 kDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas