APC Anti-Mouse CD8a Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Mouse splenocytes are stained  with Anti-Mouse CD8a Monoclonal Antibody(APC Conjugated)[Used at 0.01 μg/10<sup>6</sup> cells dilution](filled gray histogram). Unstained splenocytes (blank black histogram) are used as control.
Mouse splenocytes are stained with Anti-Mouse CD8a Monoclonal Antibody(APC Conjugated)[Used at 0.01 μg/106 cells dilution](filled gray histogram). Unstained splenocytes (blank black histogram) are used as control.
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Overview

Circle
Product Name
APC Anti-Mouse CD8a Antibody
Catalog Number
RD21262F
Clonality
Monoclonal
Isotype
Rat IgG2a, κ
Host
Rat

Background

Circle
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Immunogen Information

Circle
Gene ID
12525
Swissprot
P01731
Synonyms
T-cell surface glycoprotein CD8 alpha chainCD8AT-lymphocyte differentiation antigen T8/Leu-2MAL

Applications

Circle
Reactivity
Mouse
Tested Applications
FCM
Conjugation
APC
Concentration
0.2 mg/mL
Storage Buffer
PBS with 0.05% Proclin300, 1% BSA
Storage Instructions
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.

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