ARIH2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD219064A
Western blot analysis of Human heart tissue lysate using ARIH2 Polyclonal Antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded Human tonsil tissue using ARIH2 Polyclonal Antibody at dilution of 1:80(×200)
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ARIH2 Polyclonal Antibody at dilution of 1:80(×200)
Product Name
ARIH2 Polyclonal Antibody
Catalog Number
RD219064A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
TRIAD1, also known as ARIH2 (ariadne homolog 2) or ARI2, is a 493 amino acid protein that contains one IBR-type zinc finger and two RING-type zinc fingers and belongs to the ariadne subfamily of RBR proteins. Localized to the nucleus, TRIAD1 interacts with UBE2L3 and is thought to act as an E3 ubiquitin-protein ligase, functioning to accept ubiquitin from E2 ubiquitin-conjugating enzymes and transfer the acquired ubiquitin residue to target substrates. TRIAD1 is subject to post-translational DNA damage-dependent phosphorylation, probably by ATM or ATR. The gene encoding TRIAD1 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci.
Immunogen Information
Immunogen
Fusion protein of human ARIH2
Swissprot
O95376
Synonyms
HT005all trans retinoic acid inducible RING fingerAri 2Ari-2ARI2ARI2Ariadne 2Ariadne 2 protein homologariadne homolog 2 (Drosophila)ariadne homolog 2Ariadne RBR E3 ubiquitin protein ligase 2ARIH 2Arih2E3 ubiquitin protein ligase ARIH2E3 ubiq
Calculated MW
58 kDa
Observed MW
Refer to figures
Gene Accession
BC000422
Applications
Reactivity
Human, Mouse
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:1000-1:5000, IHC 1:50-1:300, ELISA 1:5000-1:10000
Concentration
1.38 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas