AST Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90743A
Western blot with AST Polyclonal antibody at dilution of 1:1000.lane 1:HeLa whole cell lysate,lane 2:HEK-293T whole cell lysate,lane 3:HepG2 whole cell lysate,lane 4:NIH-3T3 whole cell lysate,lane 5:C2C12 whole cell lysate,lane 6:Mouse liver,lane 7:Mouse brain,lane 8:Rat liver,lane 9:Rat brain
Product Name
AST Polyclonal Antibody
Catalog Number
RD90743A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle,it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol,and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
Immunogen Information
Immunogen
Recombinant Mouse AST protein expressed by E.coli
Swissprot
P05202
Synonyms
Aspartate aminotransferasemitochondrialmAspATFatty acid-binding protein (FABP-1)Glutamate oxaloacetate transaminase 2
Kynurenine aminotransferase 4Kynurenine aminotransferase IVKynurenine--oxoglutarate transaminase 4Kynurenine--oxoglutarate transam
Calculated MW
16kDa
Observed MW
16kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.