ATF4 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86732A
Western blot analysis of extracts of HeLa cells using ATF4 Polyclonal Antibody at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours.
Western blot analysis of extracts of HeLa cells using ATF4 Polyclonal Antibody at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours.
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Overview

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Product Name
ATF4 Polyclonal Antibody
Catalog Number
RD86732A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.

Immunogen Information

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Immunogen
Recombinant fusion protein of human ATF4
Gene ID
468
Swissprot
P18848
Synonyms
ATF4CREB-2CREB2TAXREB67TXREB
Calculated MW
19kDa/21kDa
Observed MW
27KDa

Applications

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Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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