For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86732A
Western blot analysis of extracts of HeLa cells using ATF4 Polyclonal Antibody at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours.
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.
Immunogen Information
Immunogen
Recombinant fusion protein of human ATF4
Gene ID
468
Swissprot
P18848
Synonyms
ATF4CREB-2CREB2TAXREB67TXREB
Calculated MW
19kDa/21kDa
Observed MW
27KDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.