ATG10 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD267863A
Western blot analysis of extracts of Mouse small intestine using ATG10 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of Mouse small intestine using ATG10 Polyclonal Antibody at dilution of 1:1000.
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Overview

Circle
Product Name
ATG10 Polyclonal Antibody
Catalog Number
RD267863A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human ATG10 (NP_113670.1).
Gene ID
83734
Swissprot
Q9H0Y0
Synonyms
ATG10APG10APG10Lpp12616
Calculated MW
14 kDa/25 kDa
Observed MW
25 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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