ATG5 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85570A
Western Blot analysis of Hela cells using ATG5 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat testis tissue using ATG5 Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Human ovarian carcinoma tissue using ATG5 Monoclonal Antibody at dilution of 1:200.
Product Name
ATG5 Monoclonal Antibody
Catalog Number
RD85570A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.
Immunogen Information
Immunogen
Recombinant Protein of ATG5
Swissprot
Q9H1Y0
Synonyms
APG 5APG 5LAPG5APG5 autophagy 5 likeAPG5 likeAPG5-likeAPG5LApoptosis specific proteinApoptosis-specific proteinASPATG 5Atg5ATG5 autophagy related 5 homologATG5Autophagy protein 5Autophagy related 5hAPG5Homolog of S Cerevisiae autophagy 5
Observed MW
55 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p
Conjugation
Unconjugated
Dilution
WB 1:500-2000, IHC 1:50-300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.