ATG5 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD75476A
Western Blot analysis of NIH/3T3 and A172 cell using ATG5 Polyclonal Antibody at dilution of 1:475
Immunohistochemistry of paraffin-embedded Human thyroid cancer using ATG5 Polyclonal Antibody at dilution of 1:40
Immunohistochemistry of paraffin-embedded Human liver cancer using ATG5 Polyclonal Antibody at dilution of 1:40
Product Name
ATG5 Polyclonal Antibody
Catalog Number
RD75476A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Autophagy protein 5is aproteinthat in humans is encoded by theATG5gene. It is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.
Immunogen Information
Immunogen
Recombinant protein of human ATG5
Swissprot
Q9H1Y0
Synonyms
APG 5APG 5LAPG5APG5 autophagy 5 likeAPG5 likeAPG5-likeAPG5LApoptosis specific proteinApoptosis-specific proteinASPATG 5Atg5ATG5 autophagy related 5 homologATG5Autophagy protein 5Autophagy related 5hAPG5Homolog of S Cerevisiae autophagy 5
Calculated MW
32 kDa
Gene Accession
BC002699
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:200
Concentration
0.4 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.