ATP5L Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD252677A
Western blot analysis of 293T cell Human fetal liver tissue lysates  using ATP5L Polyclonal Antibody at dilution of 1:500
Western blot analysis of 293T cell Human fetal liver tissue lysates using ATP5L Polyclonal Antibody at dilution of 1:500
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Overview

Circle
Product Name
ATP5L Polyclonal Antibody
Catalog Number
RD252677A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.

Immunogen Information

Circle
Immunogen
Fusion protein of human ATP5L
Swissprot
O75964
Synonyms
ATP synthase subunit gATP synthaseH+ transportingmitochondrial F1F0subunit gATP synthaseH+ transportingmitochondrial Fo complexsubunit GATP5LATP5LATPase subunit gF1Fo ATP synthase complex Fo membrane domain g subunitmitochondrial
Calculated MW
11 kDa
Observed MW
Refer to figures
Gene Accession
BC015128

Applications

Circle
Reactivity
Human, Mouse
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, ELISA 1:5000-1:10000
Concentration
1.8 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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