ATP6V1G3 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD81797A
Western blot analysis of extracts of Mouse kidney using ATP6V1G3 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of Mouse kidney using ATP6V1G3 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Mouse kidney using ATP6V1G3 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using ATP6V1G3 Polyclonal Antibody at dilution of 1:100 (40x lens).
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Overview

Circle
Product Name
ATP6V1G3 Polyclonal Antibody
Catalog Number
RD81797A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human ATP6V1G3 (NP_573569.1).
Gene ID
127124
Swissprot
Q96LB4
Synonyms
ATP6V1G3ATP6G3Vma10
Calculated MW
6 kDa/13 kDa/14 kDa
Observed MW
14 kDa

Applications

Circle
Reactivity
Mouse
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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