Avi-Tag Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86710A
Western blot of AVI-Tag fused recombinant protein with Anti-AVI mouse monoclonal antibody at 1:1000 dilution. Lane 1 : Recombinant AVI protein at 6.25ng; Lane 2 : Recombinant AVI protein at 12.5ng
Product Name
Avi-Tag Monoclonal Antibody
Catalog Number
RD86710A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG1
Host
Mouse
Background
Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. Biotinylation of the Avi-tag is enzymatically added by the E. coli biotin ligase BirA. This results in a single biotin molecule covalently attached to the lysine residue within the Avi-tag
Immunogen Information
Immunogen
Synthetic peptide corresponding to AVI tag conjugated to keyhole limpet haemocyanin.
Synonyms
Avi-Tag
Calculated MW
45kDa
Observed MW
45kDa
Applications
Reactivity
All
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:3000-1:6000
Concentration
7mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.