Avi-Tag Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86618A
Western blotting of AVI-Tag fused recombinant protein with Anti-AVI rabbit polyclonal antibody at dilution of lane 1: 1:10000, lane 2: 1:5000, lane 3 1:2000.
Product Name
Avi-Tag Polyclonal Antibody
Catalog Number
RD86618A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. Biotinylation of the Avi-tag is enzymatically added by the E. coli biotin ligase BirA. This results in a single biotin molecule covalently attached to the lysine residue within the Avi-tag
Immunogen Information
Immunogen
Synthetic peptide corresponding to AVI tag conjugated to keyhole limpet haemocyanin.
Synonyms
Avi-Tag
Calculated MW
55kDa
Observed MW
55kDa
Applications
Reactivity
All
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:2000-1:5000
Concentration
1.5mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.