Dilution
Immunohistochemistry in frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:100-200
Western Blotting: 1:2000-4000(Enhanced chemiluminescent detection )
Western Blotting: 1:200-400(DAB detection)
Direct ELISA: 1:10000-100000(TMB detection
Concentration
0.2 mg/mL(specific antibody purification≥99%)
Storage Buffer
PBS with 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.