B7-H3/CD276 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86800A
Western blot analysis of extracts of U-87MG cells using CD276/B7-H3 Polyclonal Antibody at 1:3000 dilution.
Immunohistochemistry of paraffin-embedded Human tonsil using CD276/B7-H3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human liver cancer using CD276/B7-H3 Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Name
B7-H3/CD276 Polyclonal Antibody
Catalog Number
RD86800A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human B7-H3/CD276
Gene ID
80381
Swissprot
Q5ZPR3
Synonyms
CD2764Ig-B7-H3B7-H3B7H3B7RP-2
Calculated MW
28kDa/40kDa
Observed MW
35kDa/55kDa/60KDa
Applications
Reactivity
Human
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas