BAP1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD267774A
Western blot analysis of extracts of various cell lines using BAP1 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human breast using BAP1 Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded Human breast cancer using BAP1 Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunofluorescence analysis of U-2 OS cells using BAP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
BAP1 Polyclonal Antibody
Catalog Number
RD267774A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma.
Immunogen Information
Immunogen
Recombinant fusion protein of human BAP1 (NP_004647.1).
Gene ID
8314
Swissprot
Q92560
Synonyms
BAP1HUCEP-13UCHL2hucep-6
Calculated MW
80 kDa
Observed MW
80 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas