Bcdin3d Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of extracts of MCF7 cells using Bcdin3d Polyclonal Antibody at 1:500 dilution.
Immunofluorescence analysis of NIH/3T3 cells using Bcdin3d Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Product Name
Bcdin3d Polyclonal Antibody
Catalog Number
RD89348A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His, acting as a capping enzyme by protecting tRNA(His from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145.
Immunogen Information
Immunogen
Recombinant fusion protein of mouse Bcdin3d
Gene ID
75284
Swissprot
Q91YP1
Synonyms
AV1387484930556P03Rik
Calculated MW
125kDa
Observed MW
126KDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.