Bcl-2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD79974A
Western blot analysis of extracts of HL-60 cells using Bcl-2 Polyclonal Antibody at dilution of 1:500.
Western blot analysis of extracts of various cells using Bcl-2 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human lung cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse testis using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
Bcl-2 Polyclonal Antibody
Catalog Number
RD79974A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
A synthetic peptide of human Bcl-2
Gene ID
596
Swissprot
P10415
Synonyms
Bcl-2PPP1R50BCL2
Calculated MW
22 kDa/26 kDa
Observed MW
24 kDa/26 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas