Bcl-2 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD82776A
Western blot analysis of extracts of various cell lines using Bcl-2 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using Bcl-2 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of L929 cells using Bcl-2 Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
Bcl-2 Polyclonal Antibody
Catalog Number
RD82776A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Immunogen Information

Circle
Immunogen
A synthetic peptide of human Bcl-2 (NP_000624.2).
Gene ID
596
Swissprot
P10415
Synonyms
Bcl-2PPP1R50BCL2
Calculated MW
22 kDa/26 kDa
Observed MW
27 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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