Bcl-2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90174A
Western blot analysis of extracts of Mouse lung using Bcl-2 Polyclonal Antibody at 1:500 dilution.
Western blot analysis of extracts of various cell lines using Bcl-2 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Bcl-2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Bcl-2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A-549 cells using Bcl-2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
Bcl-2 Polyclonal Antibody
Catalog Number
RD90174A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human Bcl-2
Gene ID
596
Swissprot
P10415
Synonyms
Bcl-2PPP1R50BCL2
Calculated MW
187kDa
Observed MW
115kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas