For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90078A
Western blot analysis of extracts of various cell lines using Beclin 1 Polyclonal Antibody at 1:1000 dilution.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Beclin 1 Polyclonal Antibody.Western blot was performed from the immunoprecipitate using Beclin 1 Polyclonal Antibody at a dilition of 1 :1000.
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human Beclin 1
Gene ID
8678
Swissprot
Q14457
Synonyms
ATG6VPS30beclin1Beclin 1BECN1beclin-1
Calculated MW
46kDa
Observed MW
46KDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.